5,6-dichlororibofuranosylbenzimidazole (DRB) is phosphorylated in salivary gland cells of Chironomus tentans

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Kinetic analysis of uptake and phosphorylation of 5,6-dichlororibofuranosylbenzimidazole (DRB) by salivary gland cells of Chironomus tentans.

The kinetics of transport and phosphorylation of the nucleoside analogue 5,6-dichloro-l-~-~-ribofuranosylbenzimidazole (DRB) and the nature of monophosphate metabolites obtained were studied in salivary gland cells of Chironomw tentans. DRB, labeled by exchange with tritiated water, is transported across the plasma membrane, but only monophosphate derivatives emerge in the ethanol-soluble cell...

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Nucleolar-derived Ribonucleic Acid in Chromosomes, Nuclear Sap, and Cytoplasm of Chironomus Tentans Salivary Gland Cells

The distribution of monodisperse high molecular weight RNA (38, 30, 28, 23, and 18S RNA) was studied in the salivary gland cells of Chironomus tentans. RNA labeled in vitro and in vivo with tritiated cytidine and uridine was isolated from microdissected nucleoli, chromosomes, nuclear sap, and cytoplasm and analyzed by electrophoresis on agarose-acrylamide composite gels. As shown earlier, the n...

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Sources of Larval Salivary Gland Secretion in the Dipteran Chironomus Tentans

The soluble proteins in the hemolymph, the salivary gland, and the salivary secretion of fourth instar Chironomus tentans were examined by disc electrophoresis in acrylamide gels. Of the 11 protein fractions detected in buffered saline extracts of the gland, 10 are present also in the hemolymph. Amino acid isotope incorporation experiments indicate that the protein fractions shared by the saliv...

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A Review of Signet Ring cells in Salivary Gland Tumors

Objectives: Signet Ring Cells (SRCs) are often seen in gastrointestinal, breast, bile duct and lung invasive carcinomas. Observing these cells in benign and malignant lesions of the salivary glands is not common. Signet-ring cell adenocarcinoma is rare and is commonly seen in the secondary salivary glands. Although, the presence of SRCs in other neoplasms of the body is usually associated with ...

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Effects of Α-amanitin on in Vitro Labeling of Rna from Defined Nuclear Components in Salivary Gland Cells from Chironomus Tentans

The effect of alpha-amanitin on nucleoside labeling of RNA in nucleoli, chromosomes, nuclear sap, and cytoplasm from Chironomus tentans salivary gland cells was investigated by radioautography and gel electrophoresis. Preribosomal RNA formation and processing in the nucleolus was not measurably influenced by the drug, and both 28 S and 18 S ribosomal RNA were transferred to the cytoplasm. In th...

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ژورنال

عنوان ژورنال: FEBS Letters

سال: 1979

ISSN: 0014-5793

DOI: 10.1016/0014-5793(79)80424-x